The Amazing Spider-Man #32

January 1966

With his Aunt May gravely ill in the hospital, Spider-Man fights as never before!

Dr. Octopus
Revealed as the "Master Planner" in this issue, Doc Oc is aiming to steal atomic equipment
Curtis Connors
A.K.A. "The Lizard," tells Spidey that a new serum, ISO-36, can cure Aunt May
J. Jonah Jameson
Publisher of the Daily Bugle and Spidey-hater
Betty Brant
JJJ's secretary, whom Peter Parker has a secret crush on, though that crush will soon be replaced by his first true love, Gwendolyn Stacy.
Frederick Foswell
Reporter at the Daily Bugle, and sometimes friend of Spider-Man
Aunt May Parker
She just lies there in this issue