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Colloquium - Dowell

Individual Genomes to Individual Differences
Massachusetts Institute of Technology
2/26/2009
3:30pm-4:30pm

We have conducted a functional comparison of two Saccharomyces cerevisiae yeast strains that exhibit dramatically different phenotypic behaviors despite being approximately as close in evolutionary distance as two individual humans. We sequenced, annotated, transcription profiled, and constructed a complete deletion library (functional analysis) for the Sigma1278b strain. By comparison to the yeast reference strain (S288C) we both obtain mechanistic explanations for known differences between the strains and accurately predict additional phenotypes.

A number of the observed genomic differences between Sigma1278b and S288C have implications for cell surface phenotypes. The (mostly) subtelomeric FLO genes confer a number of cell-surface phenotypes that we have previously shown to be under both genetic and epigenetic control. We identify new FLO genes within the Sigma1278b genome. Furthermore, our whole genome tiling expression data identifies competitive non-coding intergenic transcripts near a number of the FLO genes in Sigma1278b. A detailed study of these transcripts at the FLO11 locus sheds light on the complex regulatory relationship between transcription factors, chromatin remodelers, and transcription.

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